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伯优®细胞核分离试剂盒
伯优® 科研专用

伯优®细胞核分离试剂盒

伯优®Nuclei Isolation Kit

产品货号
产品规格
产品用途 本产品专为从动物组织中分离高纯度的单细胞核而设计。
保存条件 2~8℃避光保存
目录价 3199
数量
说明书 在线咨询

产品简介

本产品专为从动物组织中分离高纯度的单细胞核而设计。组织通过匀浆、裂解细胞膜等步骤释放完整细胞核，同时维持核膜稳定性及染色质空间结构，优化的密度梯度离心技术可进一步去除细胞碎片和杂质，从而可满足下游单细胞组学、表观遗传学等前沿研究领域对细胞核的质量要求。

 产品应用

制备的细胞核悬液可用于核转录组学（如 snRNA-seq/bulk RNA-seq），表观遗传学（如scATAC- seq/bulk ATAC- seq，CUT&Tag）等研究。

 竞品对比测试

伯优®细胞核分离试剂盒单页-03

结果图片

伯优®细胞核分离试剂盒单页-01

应用实例

伯优®细胞核分离试剂盒已成功应用于近20个物种的细胞核分离，涵盖人、小鼠、大鼠等哺乳动物及扇贝、海葵等无脊椎动物。在组织适配性方面，本试剂盒已用于包括脑、肝脏、心脏、肺、肾、脾及各类肿瘤等200余种组织，累计20,000余例样本的细胞核悬液制备。为单细胞核RNA测序提供了稳定可靠的细胞核分离解决方案。

单细胞核RNA测序实测组织类型

伯优®细胞核分离试剂盒单页-02



This product is specially designed to isolate high-purity single nuclei from animal tissues. Intact nuclei are released through tissue homogenization and cell membrane lysis, while the nuclear membrane stability and spatial structure of chromatin are well preserved. The optimized density gradient centrifugation effectively removes cell debris and contaminants, enabling the nuclei to meet strict quality requirements for cutting-edge research including single-cell omics and epigenetics.

Applications

The prepared nuclear suspension is applicable to nuclear transcriptomics (e.g., snRNA-seq, bulk RNA-seq) and epigenetics research (e.g., scATAC-seq, bulk ATAC-seq, CUT&Tag).

Microscopy Images

52009-1

Application Cases

Boyou® Nucleus Isolation Kit has been successfully used for nucleus extraction from nearly 20 species, including mammals such as humans, mice and rats, as well as invertebrates like scallops and sea anemones. It is compatible with over 200 tissue types including brain, liver, heart, lung, kidney, spleen and various tumor tissues, with more than 20,000 sample preparations completed so far. It provides a stable and reliable solution for single-nucleus RNA sequencing.

Tested Tissue Types for Single-Nucleus RNA Sequencing

52009-2


应用文献

IF 8 npj Precision Oncology 2026
Single-nucleus sequencing dissects the malignant change of early lung adenocarcinoma: SMAD3 suppresses antitumor T cell immunity via ICAM1
IF 4.3 Frontiers in Cell and Developmental Biology 2026
Cardiomyocyte-derived BDNF restricts cardiac fibrosis by decreasing the activity of the TGF-β/Smad2/3 pathway and increasing Smad7 expression
IF 42.5 CELL 2026
B cell deficiency limits exercise capacity by remodeling liver glutamate metabolism
IF 9.4 Genes & Diseases 2026
Rac1 impairs retinal axon degeneration via Kif2a in a mouse model of chronic ocular hypertension
IF 15.7 Nature Communications 2026
Impact of NPAS2 on mPFC dopamine synthesis and nap behavior
IF 2.4 GENE 2026
Differential early tubular injury in minimal Change disease and Focal segmental Glomerulosclerosis: A Multifaceted analysis
IF 15.7 Nature Communications 2026
Gut microbiota-derived butyrate primes systemic immunity in honey bees by mediating lipid metabolic reprogramming
IF 25.9 CELL RESEARCH 2026
Mitochondrial double-stranded RNA drives aging-associated cognitive decline
IF 10.1 Journal of Neuroinflammation 2025
NEO1 modulates the A1 astrocyte polarization in subarachnoid hemorrhage through the cPLA2-MAVS signaling pathway
IF 6.9 Scientific Data 2025
Comprehensive snRNA-Seq Datasets of Human and Mouse Podocytopathy Integrated with GWAS of Microalbuminuria
IF 7.9 Clinical and Translational Medicine 2025
Proteogenomic characterisation of primary oral cancer unveils extracellular matrix remodelling and immunosuppressive microenvironment linked to lymph node metastasis
IF 4.6 Frontiers in Cell and Developmental Biology 2025
snRNA-seq reveals subcutaneous white adipose tissue remodeling upon return to thermoneutrality after cold stimulation
IF 7.9 JOURNAL OF HEADACHE AND PAIN 2025
Selective loss and transcriptional reprogramming of Nox4+ GABAergic neurons in the trigeminal nucleus caudalis of NTG-induced chronic migraine model
IF 15.7 Nature Communications 2025
Fosl2 facilitates chromatin accessibility to determine developmental events during follicular maturation
IF 15 NEURON 2025
Segregated supramammillary-dentate gyrus circuits modulate cognitive and affective function in healthy and Alzheimer’s disease model mice
IF 10.1 International Journal of Surgery 2025
Neuron-specific transcriptomic dysregulation in methotrexate-induced cognitive impairment revealed by SnRNA-seq
IF 10.1 MOLECULAR PSYCHIATRY 2025
Bcl11a deficiency in cerebellar Purkinje cells causes ataxia and autistic-like behavior by altering Vav3
IF 7.5 Journal of Translational Medicine 2025
A multi-omic single-cell landscape reveals transcription and epigenetic regulatory features of t(8;21) AML
IF 48.5 NATURE 2025
Constitutively active glucagon receptor drives high blood glucose in birds
IF 6.2 ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2025
Characterizing microglial heterogeneity in autophagy impairment of Paraquat-induced Parkinson’s disease-like neurodegeneration
IF 20.5 Nature Microbiology 2025
Integrating microbial GWAS and single-cell transcriptomics reveals associations between host cell populations and the gut microbiome
IF 7.9 Clinical and Translational Medicine 2025
Proteogenomic characterisation of primary oral cancer unveils extracellular matrix remodelling and immunosuppressive microenvironment linked to lymph node metastasis
IF 3.9 Frontiers in Endocrinology 2025
Single-nucleus RNA sequencing reveals dynamic changes in the microenvironment of visceral adipose tissue and metabolic characteristics after cold exposure
IF 14.3 Advanced Science 2025
Radiotherapy-Associated Cellular Senescence and EMT Alterations Contribute to Distinct Disease Relapse Patterns in Locally Advanced Cervical Cancer
IF 6.1 Journal of Translational Medicine 2025
Single-cell atlas reveals multi-faced responses of losartan on tubular mitochondria in diabetic kidney disease
IF 1.3 STAR Protocols 2025
Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics
IF 14.3 Advanced Science 2024
Neuroligin 1 Regulates Autistic-Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor-Expressing Medium Spiny Neurons
IF 9.4 PNAS 2024
Pathogenic mechanism and therapeutic intervention of impaired N7-methylguanosine (m7G) tRNA modification
IF 6.2 ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2024
Brain single-cell transcriptomics highlights comorbidity-related cell type-specific changes of Parkinson’s disease with major depressive disorder after paraquat exposure
IF 7.5 Cell Reports 2024
RAC1 inhibition ameliorates IBSP-induced bone metastasis in lung adenocarcinoma
IF 7.7 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2024
GSK3 regulation Wnt/β-catenin signaling affects adipogenesis in bovine skeletal muscle fibro/adipogenic progenitors
IF 7.5 Cell Reports 2024
The architecture of silk-secreting organs during the final larval stage of silkworms revealed by single-nucleus and spatial transcriptomics
IF 6.1 Neural Regeneration Research 2024
Spatial transcriptomics combined with single-nucleus RNA sequencing reveals glial cell heterogeneity in the human spinal cord
IF 4.8 Frontiers in Aging Neuroscience 2024
Single-cell sequencing of the substantia nigra reveals microglial activation in a model of MPTP
IF 7.7 COMPUTERS IN BIOLOGY AND MEDICINE 2024
Dissecting microenvironment in cystadenomas and hepatic cysts based on single nucleus RNA-sequencing data
IF 6.8 ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2024
Single-cell RNA-sequencing of cellular heterogeneity and pathogenic mechanisms in paraquat-induced Parkinson's disease with depression
IF 12.4 Theranostics 2024
An advanced comprehensive muti-cell-type-specific model for predicting anti-PD-1 therapeutic effect in melanoma
IF 15.1 Advanced Science 2023
Single-Nucleus Transcriptome Profiling of Locally Advanced Cervical Squamous Cell Cancer Identifies Neural-Like Progenitor Program Associated with the Efficacy of Radiotherapy
IF 4.6 JOURNAL OF DERMATOLOGICAL SCIENCE 2023
Updated skin transcriptomic atlas depicted by reciprocal contribution of single-nucleus RNA sequencing and single-cell RNA sequencing
IF 13.437 MOLECULAR PSYCHIATRY 2022
Development of opioid-induced hyperalgesia depends on reactive astrocytes controlled by Wnt5a signaling
IF 14.224 Journal Of Hazardous Materials 2022
Brain single-nucleus transcriptomics highlights that polystyrene nanoplastics potentially induce Parkinson’s Disease-like neurodegeneration by causing energy metabolism disorders in mice
IF 6.4 Elife 2021
Single-nucleus transcriptomic atlas of spinal cord neuron in human

产品问答

Q: 52009和52201都是针对组织样本通用款，两款抽核试剂盒流程及原理、起始量方面有什么区别？
A: 两款试剂盒基本操作流程均为：组织匀浆及细胞裂解、碎片去除、清洗、重悬细胞核。52009通过密度梯度法去除碎片及杂质，获得的细胞核纯度高；52201柱提法通过过滤柱及碎片去除液去除碎片及杂质，操作流程短，得率高。建议起始量为20-50mg，52201兼容10mg以下低起始量。


Q: 52009试剂盒单次测试需要多少组织？组织研磨用什么方法？
A：组织起始量建议20-50mg，研磨方法可根据组织类型选择电动匀浆仪研磨或研磨杵手动研磨。


Q: 小鼠肝抽核，后续做ATAC-seq。新鲜与冰冻组织后续是否有区别？是否有方法能保存新鲜组织？
A: 相对来说新鲜组织更好，冰冻组织液也可以做。单细胞测序组织保存液（伯优，21903）可在72h内保持组织细胞活性。


Q: 52009处理冰冻组织和新鲜组织都可以吗？
A: 新鲜和冰冻组织都可以的。


Q: 肿瘤样本，后续单细胞转录组，推荐用什么研磨匀浆方法？
A:推荐使用电动匀浆仪（Omni TH 手持/台式两用均质器）低转速研磨5-10s。


Q: 分离细胞核的组织可以冻融几次？
A: 分离的细胞核不建议冻融，建议半小时内进行后续实验。


Q: 某些样本匀浆无法完全匀浆，如何改善？
A: 可先用剪刀剪碎组织，再进行匀浆。大部分组织匀浆即可，过度匀浆也会影响细胞核形态及得率。


Q: Both 52009 and 52201 are general kits for tissue samples. What are the differences between them in workflow, principle and initial sample amount?
A: The basic workflow of both kits includes tissue homogenization & cell lysis, debris removal, washing and nucleus resuspension. Kit 52009 removes debris and impurities via density gradient method, delivering nuclei with high purity. Kit 52201 adopts column-based purification using filter columns and debris removal solution, featuring a shorter workflow and higher yield. The recommended initial sample amount is 20–50 mg. Kit 52201 is also compatible with low-input samples below 10 mg.


Q: How much tissue is required for a single test with Kit 52009? What methods are available for tissue grinding?
A: The recommended initial tissue amount is 20–50 mg. Either an electric homogenizer or a manual pestle can be used for grinding, depending on tissue type.


Q: For nucleus extraction from mouse liver samples followed by ATAC-seq, are there any differences between fresh and frozen tissues? Is there a solution to preserve fresh tissues?
A: Fresh tissues are preferred, while frozen tissues are also applicable. Single-cell Tissue Preservation Solution (Boyou, Cat. 21903) can maintain cellular activity for up to 72 hours.


Q: Can Kit 52009 be used for both frozen and fresh tissues?
A: Yes, it works for both fresh and frozen tissues.


Q: For tumor samples intended for subsequent single-cell RNA-seq, which grinding and homogenization method is recommended?
A: Use an electric homogenizer (Omni TH Handheld/Benchtop Homogenizer) at low speed for 5–10 seconds.


Q: How many freeze-thaw cycles are allowed for isolated nuclei?
A: Repeated freeze-thaw of isolated nuclei is not recommended. Subsequent experiments should be performed within half an hour.


Q: How to improve incomplete tissue homogenization for certain samples?
A: Cut the tissue into small pieces with scissors prior to homogenization. Moderate homogenization is sufficient; excessive homogenization will impair nucleus morphology and yield.

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