产品简介
产品简介
本产品适用于人和动物新鲜及冻存组织样本的细胞核分离提取。利用离心管柱技术可在20分钟内，快速分离出完整的细胞核。优化 的碎片去除步骤有效去除组织碎片和杂质，避免细胞质的污染的同时，可维持细胞核膜的完整和染色质的空间结构稳定。
产品应用
| 制备的细胞核悬液可用于 | 1、表观遗传学研究（如scATAC- seq/bulk ATAC- seq，CUT&Tag）； |
| 2、核转录组学研究（如 snRNA-seq/bulk RNA-seq）等相关实验。 |
试剂盒对比
| 试剂盒 | 分离方法 | 组织研磨方法 | 单个样本操作时间 | 是否去细胞碎片 | 细胞核质量 | 抽核得率 |
| 伯优细胞核分离试剂盒-柱提法 | 离心管柱法 | 电动/研杵匀浆 | 20分钟 | 是 | 高 | 高 |
| 伯优细胞核分离试剂盒 | 密度梯度离心法 | 电动/研杵匀浆 | 40分钟 | 是 | 高 | 较高 |
镜检图
 小鼠脑组织 |  小鼠肺组织 |  小鼠肝组织 |
 人脑组织 |  人肺组织 |  人肝组织 |
Product Description
This kit is designed for the isolation and extraction of cell nuclei from fresh and cryopreserved human and animal tissue samples. Adopting advanced centrifugal column technology, it enables rapid and efficient isolation of intact cell nuclei within only 20 minutes per sample.
The optimized debris removal procedure effectively eliminates tissue fragments and cytoplasmic contaminants, thoroughly avoiding cytoplasmic pollution. Meanwhile, it perfectly maintains the integrity of the nuclear membrane and preserves the spatial structural stability of chromatin, providing high-quality nuclear samples for downstream omics research.
Product Applications
| The purified nuclear suspension obtained by this kit is suitable for a variety of cutting-edge omics experiments: | 1. Epigenetic research: scATAC-seq, bulk ATAC-seq, CUT&Tag and other related epigenetic profiling experiments; |
| 2. Nuclear transcriptomics research: snRNA-seq, bulk RNA-seq and other nuclear transcriptome sequencing analyses. |
Kit Performance Comparison
Kit Name | Isolation Method | Tissue Homogenization Method | Processing Time per Sample | Debris Removal | Nucleus Quality | Nucleus Yield |
|---|---|---|---|---|---|---|
Boyou Nucleus Isolation Kit (Column-based Method) | Centrifugal Column Method | Electric homogenizer / Pestle homogenization | 20 minutes | Yes | High | High |
Boyou Nucleus Isolation Kit (Density Gradient Method) | Density Gradient Centrifugation | Electric homogenizer / Pestle homogenization | 40 minutes | Yes | High | Relatively High |
Micrograph
 mouse brain tissue |  mouse lung tissue |  mouse liver tissue |
 human brain tissue |  human lung tissue |  human liver tissue |
